ovarian cancer cell line ov90  (ATCC)

Journal: bioRxiv

Article Title: Abolishing respiratory complex I decreases in vivo growth of high grade serous ovarian cancer cells and sensitizes to anti-angiogenic therapy

doi: 10.64898/2026.02.28.708681

Figure Lengend Snippet: (a) HIF1a western blot analysis in representative OV-90 +/+ and OV90 −/− xenografts (n=3 per group). Calreticulin was used as loading control. (b) Gene expression of HIF1 targets evaluated by qRT-PCR in OV-90 +/+ (n=5) and OV-90 −/− (n=6) xenografts. Data were log transformed prior to statistical analysis. (c) Masson’s trichrome staining of OV-90 +/+ and OV90 −/− xenografts, with respective quantification of the stroma (collagen areas stained in blue). Representative images are shown with black squares indicating the insets of the areas shown at higher magnification. (d) Immunofluorescent staining of endomucin (Endo) and Smooth Muscle Actin (SMA) in OV-90 +/+ and OV-90 −/− xenografts, with nuclei stained with DAPI, scale bar 200 µm. White squares indicate the insets of the areas shown at higher magnification. Representative images are shown, together with quantification of total (Endo + ) and pericyte negative (Endo + SMA − ) vessels. (e) Ultrasound images of OV-90 +/+ and OV-90 −/− xenografts. Incoming (red) and outcoming (blue) blood flows are shown. The graph indicates the percentage of the blood flow areas detected within each tumor (n=3 per group). One-tailed t-test was used to compare averages between the groups. (f) Immunofluorescent staining of F4/80 + macrophages (red) in OV-90 +/+ and OV-90 −/− xenografts. White squares indicate the insets of the areas shown at higher magnification. The arrows indicate F4/80 + cells. Nuclei are stained with DAPI (blue), scale bar = 20 µm. For all panels, data in graphs are represented as mean ± SEM and p-value is indicated.

Article Snippet: The human HGSOC cell line OV90 was purchased from ATCC® (Manassas, VA, USA #CRL-3585).

Techniques: Western Blot, Control, Gene Expression, Quantitative RT-PCR, Transformation Assay, Staining, One-tailed Test

Journal: Cell Death & Disease

Article Title: Splice-switching of the oncogenic BCS1L isoform suppresses ovarian cancer progression by disrupting mitochondrial function

doi: 10.1038/s41419-026-08495-6

Figure Lengend Snippet: A Venn diagram of 53 BCS1L -bound splicing factors from the BCS1L RNA pulldown-MS in A2780 cells and 24 genes regulating BCS1L splicing derived from the TCGA-OV datasets (Pearson’s R > 0.15, p < 0.001). B Volcano plots of differentially expressed BCS1L -bound splicing factors between the TCGA-OV ( n = 374) dataset and the normal tissue in GTEx-ovary ( n = 180) datasets. |Pearson R | > 0.1 and p -value > 0.05 were considered significant. Positive correlations are shown in red, and negative correlations are shown in blue. C Relative BCS1L-L/BCS1L-S transcript expression was measured by qPCR in A2780 cells transfected with siRNAs targeting YBX1 , PUF60 , HSPA8 , USP39 , HNRNPF , SF3B4 , SNRPC , and SNRNP40 or a negative control for 48 h. D Sashimi plots of the alternative splicing pattern and USP39 direct binding sites in BCS1L were created with IGV using RNA-seq and RIP-seq data in A2780 cells. The light blue region indicates the alternative exon and the USP39-binding sites. E Semi-quantitative RT-PCR was performed to validate alternative splicing events in A2780 cells with USP39 knockdown using isoform-specific primers. F The relative expression ratio of BCS1L-L/BCS1L-S was analyzed in A2780 cells with USP39 depletion using isoform-specific primers. G Schematic diagram of the BCS1L minigene structure and alternative splicing products. H The expression of BCS1L minigene transcripts in 293T cells with USP39 depletion using different USP39 siRNAs as measured by semi-quantitative RT-PCR. I The expression of BCS1L minigene transcripts in 293T cells transfected with USP39 expression plasmids was measured by semi-quantitative RT-PCR. J The interaction between USP39 and BCS1L RNA was validated by RIP-qPCR of A2780 cells overexpressing USP39. U6snRNA served as the positive control. n = 3 biologically independent experiments. K RNA pull-down assay showing the interaction between the BCS1L RNA and USP39 protein in A2780 cells. Androgen receptor 3´-UTR RNA was used as the positive control and poly (A) 25 RNA was used as the negative control. L The protein expression of BCS1L-L and BCS1L-S was determined by western blot in A2780, HEY, and OV90 cells with USP39 knockdown and in OVCAR3 cells with USP39 overexpression. The top band at 48 kDa indicates BCS1L-L, and the bottom band at 34 kDa indicates BCS1L-S. M Representative confocal images of control A2780 cells and A2780 cells with USP39 knockdown showing co-localization of BCS1L (green) and COX4 (red). DAPI was used to visualize nuclei. Scale bars, 10 µm. Images are representative of at least three independent experiments. Co-localization coefficients, including Pearson correlation coefficient and Mander’s coefficient were quantified by Image J ( n = 50 cells for control, n = 50 cells for USP39 knockdown). The p -value ( C , F , J , M ) was obtained by Student’s unpaired t -test and and the results are presented as the mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Article Snippet: Human ovarian cancer cell lines OV90, SKOV3, OVCAR3, OVCAR8, and CAOV3 were purchased from the American Type Culture Collection.

Techniques: Derivative Assay, Expressing, Transfection, Negative Control, Alternative Splicing, Binding Assay, RNA Sequencing, Quantitative RT-PCR, Knockdown, Positive Control, Pull Down Assay, Western Blot, Over Expression, Control

Journal: bioRxiv

Article Title: Cancer-Associated Mesothelial Cells Drive Immune Escape and Therapy Resistance in Ovarian Cancer

doi: 10.64898/2026.01.07.698232

Figure Lengend Snippet: A) Quantitative PCR (qPCR) analysis of Upk3b and Serpinb2 expression in immortalized mesothelial cells (iMC) stimulated with KPCA-conditioned media and Met5a stimulated with patient D HGSOC media (ptD) or Ov90 ovarian cancer cell line in a time-dependent manner (0-96hrs). B) Western blot analysis of UPK3B and SERPINB2 expression in iMC treated with KPCA-condioned culture medium at 0h, 24h, 48h, 72h and 96h (left panel) and Met5A stimulated with conditioned media from ptD/Ov90 cell-lines versus unstimulated controls at 72h (right panel). C) Immunofluorescence analysis of UPK3B and SERPINB2 in iMC and Met5A comparing conditions stimulated with either KPCA-conditioned media or ptD/Ov90 media versus unstimulated controls at 72 h. D) Immunofluorescence staining for ACTA2 and COL4A1 in both primary MCs and immortalized MCs (iMCs) after 96 hours of exposure to KPCA-conditioned media

Article Snippet: The KPCA cell lines were developed and described in Iyer et al. and the OV90 cells lines of malignant papillary serous adenocarcinoma were purchased from ATCC.

Techniques: Real-time Polymerase Chain Reaction, Expressing, Western Blot, Immunofluorescence, Staining

Journal: NPJ Precision Oncology

Article Title: Perfusion-based ex vivo culture of frozen ovarian cancer tissues with preserved tumor microenvironment

doi: 10.1038/s41698-025-00941-6

Figure Lengend Snippet: A Representative images of haematoxylin and eosin (HE) staining of the U-CUP perfused sensitive (OV90) and resistant (OV90cis) OC cells treated with cisplatin (CDDP, 18 μM). Black squares indicate the insets of the areas shown at higher magnification. Scatter plot represents the nuclei percentage within the 4 µm section, normalized to the untreated control (UT). B Representative IF images of the U-CUP perfused sensitive (OV90) and resistant (OV90cis) cells treated with cisplatin (CDDP, 18 µM). Nuclei (DAPI, blue) and cleaved caspase 3 (cC3, red) are shown in MERGE. The single fluorescence channel is shown separately in black/white images. Scatter plot represents the percentage of cC3 + cells per 4 µm section. For all panels, data from two independent experiments ( n = 2 biological replicates for each experiment) and the mean values ±SEM are shown. QuPath was used for quantification; unpaired t-tests were applied for comparison; and p-values are shown within the graphs.

Article Snippet: The human OC OV90 cell line was purchased from the ATCC (American Type Culture Collection), and cultured at 37 °C in an incubator with 5% CO 2, using RPMI 1640 Medium (Life technologies #31870-025) supplemented with 10% FBS (Life Technologies #10270), 1% Penicillin/Streptomycin (Life Technologies #15070-063) and 2 mM L-Glutamine (Lonza BE #17-605E).

Techniques: Staining, Control, Fluorescence, Comparison

Journal: Journal for Immunotherapy of Cancer

Article Title: Universal off-the-shelf combination immunotherapy using oncolytic viruses to redirect T cell engagers to target solid tumors

doi: 10.1136/jitc-2024-011051

Figure Lengend Snippet: OV effectively delivers BCMAt to solid tumors and redirects activation and cytotoxicity of BCMA-CAR T cells and human T cells in the presence of BCMA-TCE in vitro. ( a ) Illustration of vaccinia OV with BCMAt incorporated under the control of the synthetic early promoter inserted into the J2R locus and replacing the tk gene (OVBCMAt). ( b ) Quantification of BCMAt expression on various solid tumor cell lines 24 (left) and 48 hours (right) after exposure to indicated MOI of OVBCMAt. n=2 per condition and each experiment was repeated two times. ( c–e ) Quantification of CD137 expression (left), tumor cell killing (middle), and BCMAt expression (right) assessed by flow cytometry after 24 hours of ( c ) MDA-MB-468, ( d ) SNU-16, or ( e ) OV90 tumor cells co-cultured with or without BCMA-TCE in the presence of human T cells or BCMA-CAR T cells and indicated MOI of OVBCMAt. n=2 per condition and each experiment was repeated three times. P values indicate differences between BCMA-TCE groups with PBMCs and PBMCs only determined using unpaired Student’s t-test (*p<0.05, **p<0.01, and ***p<0.005; ns=not significant). BCMAt, truncated B cell maturation antigen; CAR, chimeric antigen receptor; MOI, multiplicity of infection; OV, oncolytic virus, OVBCMAt, OV carrying the BCMAt-encoding gene; PBMC, peripheral blood mononuclear cell; TCE, T cell engager; tk, thymidine kinase; UTD, untransduced.

Article Snippet: Human ovarian cancer cell line OV90 (ATCC CRL-11732) was cultured in 1:1 vol of MCDB 105 medium (Sigma-Aldrich) and medium 199 (Gibco) containing 20% FBS and 1× AA.

Techniques: Activation Assay, In Vitro, Control, Expressing, Flow Cytometry, Cell Culture, Infection, Virus